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Lecturer(s)
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Sekaninová Jana, Mgr. Ph.D.
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Danihlík Jiří, Mgr. Ph.D.
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Course content
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Basics of Clinical Laboratory Work and Blood Collection at the Olomouc University Hospital Lipids (Spectrophotometric determination of triacylglycerols and cholesterol in blood serum) Glucose (Spectrophotometric determination of glucose in blood serum, glycemic curve) Urine parameters (Determination of basic urine parameters by test tube reactions and diagnostic strips) Toxicology (Extraction of drugs and alkaloids from urine and subsequent detection by TLC, determination of alcohol in the breath) Blood serum proteins (Spectrophotometric determination of albumin and total proteins, blood serum electrophoresis) Immunomethods (Determination of antibodies against EBV (or Borrelia) using a commercial ELISA kit, agglutination test for the determination of CRP and Antistreptolysin) Determination of nitrogenous non-protein substances (Spectrophotometric determination of urea, uric acid and creatinine) Determination of Inorganic Ions (Spectrophotometric Determination of Ca, Mg, P) Determination of iron and blood group (Spectrophotometric determination of iron in blood serum, determination of blood group by agglutination test)Determination of enzyme activity (Spectrophotometric determination of lactate dehydrogenase or amylase activity) Excursion to the laboratories of clinical biochemistry of the Olomouc University Hospital or to the Transfusion Department of the Olomouc University Hospital
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work, Group work
- Attendace
- 52 hours per semester
- Homework for Teaching
- 30 hours per semester
- Preparation for the Course Credit
- 30 hours per semester
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Learning outcomes
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Main approaches used in clinical biochemistry. Practical application of methods in clinical laboratories.
Orientation (understanding) and independent work with commercial kits for the analysis of biochemical parameters in blood serum.
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Prerequisites
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Basic kowledge from biochemistry
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Assessment methods and criteria
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Student performance, Systematic Observation of Student
100% attendance at the exercises. In case of illness or other serious reasons, the exercise can be replaced at a date agreed with the exercise leader. Submitted and recognized protocols from all exercises. Each student submits his/her own report in printed form in the next exercise at the latest. He can get 1 point for each protocol. If the report is not submitted by the deadline or if it has to be corrected repeatedly, 1 point is always deducted. The protocol must include: 1. Principle of determination 2. Results (standard deviation and relative error) 3. Evaluation/conclusion (clinical relevance) During the exercises, the student's readiness will be tested in the form of a test or oral examination. Credit will be awarded to a student who obtains 75% of the total points (tests + protocols). Who does not reach 75%, writes a credit test (can write max. 2x).
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Recommended literature
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Brodská HL, Kohout P a kol. Laboratorní vyšetření v klinické praxi. 2022.
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Kaplan and Pesce. Clinical chemistry: theory, analysis, correlation. USA: Mosby Elsevier. 2010.
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Racek J. a kol. Klinická biochemie. Praha: Galén. 2021.
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Zajoncová, L. (2013). Praktická cvičení z klinické biochemie. UP Olomouc.
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Zima T. a kol. Laboratorní diagnostika. Praha: Galén. 2017.
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