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Lecturer(s)
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Kosztyu Petr, Mgr. Ph.D.
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Zachová Kateřina, Mgr. Ph.D.
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Raška Milan, prof. Mgr. MUDr. Ph.D.
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Course content
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1. Evaluation of neutralizing antibodies 1 - preparation of pseudoviruses 2. Evaluation of neutralizing antibodies 2 - measurement of neutralizing activity using pseudoviruses 3. Evaluation of antibody affinity - SPR 4. Evaluation of antibody subclasses - fluoroenzyme assay 5. Evaluation of allergen specific antibodies - biochip ALEX 6. Multiparametric determination of leukocyte subpopulations 7. Determination of antigen-specific immune cell response of lymphocytes - proliferation assay 8. Determination of antigen-specific immune cell response of lymphocytes - detection of intracellular cytokine production 9. Measurement of immune complex formation - SPR
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Learning activities and teaching methods
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Demonstration, Laboratory Work
- Attendace
- 52 hours per semester
- Homework for Teaching
- 20 hours per semester
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Learning outcomes
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The student will learn the basic principles of preparation and performance and evaluation of selected immunological determinations by practical performance or observation.
Understanding of the principles of methods used in immunology and the ability to implement them in practice.
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Prerequisites
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By completing the KBC/ZIMU course, students will have sufficient knowledge to be able to take the KBC/CI course.
KBC/MIM and KBC/ZIMU
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Assessment methods and criteria
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Student performance
100% participation in the training, prepared protocols
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Recommended literature
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(2020). Current protocols in Immunology, in Flow cytometry. John Wiley & Sons.
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Abbas, A. K., Lichtman, A. H., Pillai, S. (2007). Cellular and Molecular Immunology. Saunders.
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Bartůňková, J., Paulík, M., a kol. (2011). Vyšetřovací metody v imunologii. Praha: Grada.
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Janeway, Ch. A., Travers, P., Walport, M. (2018). Immunobiology. Garland Science New York.
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Krejsek, J., Kopecký, O. (2004). Klinická imunologie. Nucleus HK.
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Křupka, M., Raška, M., et al. (2020). Imunologické vyšetřovací metody. LF UP Olomouc.
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