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Lecturer(s)
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Course content
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Nucleic acids and their structure. DNA transcription to RNA, types of RNA molecules, mRNA, tRNAs, rRNAs, small RNAs, antisense transcripts, gene definition and characterization, structural genes, regulatory genes, orthologous and paralogous genes, gene families, duplication and copy number variation of genes, gene variations, alternative splicing, gene evolution, transcription of repetitive DNA, regulation of gene expression, RNAi, chromatin modification. Transcriptome sequencing, identification of genes and functional domains, tools for gene prediction, Glimer, GeneMark, GeneScan. Gene expression analysis, Northern blots, cDNA, microarrays, RT-PCR, SAGE, gene databases. Gene validation and functional genomics, gene transformation, transgenesis techniques, VIGS, Agrobacterium, mutagenesis, TILLING, transposon tagging, TALEN.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming), Demonstration
- Preparation for the Exam
- 55 hours per semester
- Attendace
- 26 hours per semester
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Learning outcomes
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The course will introduce basic and advanced tools for gene identification, transcriptome sequencing, gene expression analysis and functional genomics.
After the course, student should be able to: - understand and define different types of genetic information - use tools for prediction and analysis of genes - understand gene validation and processes of trangenesis
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Prerequisites
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Successful completion of bachelor's level study requirements
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Assessment methods and criteria
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Oral exam
Successful passing of oral examination
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Recommended literature
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Gibson, G., Muse, V.S. (2009). A Primer of Genome Science, 3. vydání. Sinauer Associates, Inc., Sunderland.
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Gibson, G., Muse, V.S. (2004). A Primer of Genome Science. Sunderland.
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Kahl, G., Meksem, K. (2008). The Handbook of Plant Functional Genomics. Weinheim.
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Kole, Ch., Abbot, A.G. (2008). Principles and Practices of Plant Genomics. Enfield.
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Ondřej M., Drobník J. (2002). Transgenoze rostlin. Akademia.
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Pevsner, J. (2015). Bioinformatics and Functional Genomics. Wiley-Blackwell, Hoboken, New Jersey.
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Šmarda et al. (2005). Metody molekulární biologie. Brno.
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