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Lecturer(s)
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Course content
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Genetic mapping - linkage and association mapping, genetic markers, mapping populations, genetic mapping in human. Physical mapping - cytogenetic, optical, radiation hybrid and BAC contig maps, mapping based on chromatin conformation capture. Positional gene cloning, collinearity. Whole-genome sequencing strategies and procedures. Second- and third-generation sequencing. Sequence assembling and annotation. Single nucleotide polymorphism (SNP) identification and genotyping, high-throughput genotyping platforms. Transcriptomics - analysis of transcriptomes by DNA microarrays and RNAseq. Determination of gene function - saturation forward genetics, reverse genetics, genome editing by synthetic nucleases, approaches to identification of regulatory sequences. Genome projects in prokaryotes, plants, animals and human.
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Learning activities and teaching methods
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Dialogic Lecture (Discussion, Dialog, Brainstorming), Work with Text (with Book, Textbook)
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Learning outcomes
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Providing advanced information on genomic projects and on recent methods and approaches to studying genomes.
Student will be able to - explain and apply recent genomics techniques - orient himself in the genomics problems and lead scientific discussion in broader context of the whole discipline
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
Exam in the extent of the presented subject matter.
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Recommended literature
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Birren et al. (1997). Genome analysis - Mapping Genomes. New York.
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Gibson, G., Muse, V.S. (2004). A Primer of Genome Science. Sunderland.
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Kole, Ch., Abbot, A.G. (2008). Principles and Practices of Plant Genomics. Enfield.
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Meksem, K., Kahl, G. (2005). The Handbook of Plant Genome Mapping. Weinheim.
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Šmarda et al. (2005). Metody molekulární biologie. Brno.
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