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Lecturer(s)
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Grycová Aneta, Mgr. Ph.D.
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Navrátil Milan, prof. RNDr. CSc.
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Course content
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Viruses: definition, structure, properties and classification. Live cycle of viruses, lytic and lysogenic cycle of bacteriophage, reproductive cycle of HSV and HIV. Pathogenesis of viral infection, viruses and tumors, prevention and therapy of viral infections. Virus ecology, epidemiology of viral infections, natural focal infections, transmission and spatial/temporal spread of viruses. Origin and evolution of viruses. Economic importance of viruses. Methods of study of viruses. Definition, properties and reproduction of viroids, satellites and prions. Practical seminar: Safety and standards of work in virological laboratory. Electron microscopy in the study of viruses. Mechanical transmission of plant virus. Determination of viral antigen by double immunodiffusion. Virus detection by DAS-ELISA. Determination of IgG antibodies against varicella virus by ELISA. Determination of the concentration of phage particles by plaque method. Excursion to microbiological diagnostic laboratory.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
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Learning outcomes
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Introducing students with viruses, viroids and prions, their classification, reproduction cycle and ecology. Emphasis is placed on the ability of the student to independently and accurately realize the experiment according to written instructions.
Student is able to (after attending the course): - Describe the sub-cellular organiosms and principles of their classification (viruses, viriods, satellites, priones). - Describe the reproduction cycles of viruses. - Describe the interactions virus-host and main phases of pathogenesis. - Describe the main factors for understanding the epidemiology of viral diseases. - Perform basic experiments focused on detection of viruses by immunoenzymatic methods. - Write the protocol/report and discuss the results.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral exam
credit: Attendance at practical seminar, protocols elaboration, 70% test. Oral exam in extent of the lectures, 2 randomly selected questions.
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Recommended literature
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Bednář, M., Fraňková, V., Schindler, J., Souček, A., Vávra, J. (1996). Lékařská mikrobiologie. Marvil.
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Kůdela, V. a kol. (1989). Obecná fytopatologie. Academia Praha.
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Navrátil M. (2011). Základy virologie: Obecná virologie. Olomouc.
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Votava, M. (2005). Lékařská mikrobiologie obecná. Brno: Neptun.
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Žemla, J., Čiampor, F., Labuda, M. (1995). Špeciálna virologie. SAP, Bratislava.
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