| Course title | Practice in Molecular Biology 1 |
|---|---|
| Course code | KBB/MBCSB |
| Organizational form of instruction | Exercise |
| Level of course | Bachelor |
| Year of study | not specified |
| Semester | Summer |
| Number of ECTS credits | 3 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
|---|
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| Course content |
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1) The course introduction. Laboratory safety and rules. 2) Isolation of Plant genomic DNA. Measuring of the DNA concentration and purity, its electrophoretic separation on agarose gel. 3) Isolation of imunoglobulin fraction IgG of rabbit blood serum. Electrophoretic separation of proteins on polyacrylamide gel (PAGE), detection of their molecular weights. 4) Plasmid transformation of competent bacteries, gene transfer of resistence to antibiotics. 5) Detection of aryl hydrocarbon receptor in tumor cell lines (protein izolation, SDS-PAGE, westernblotting, immunodetection).
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| Learning activities and teaching methods |
| Dialogic Lecture (Discussion, Dialog, Brainstorming), Laboratory Work |
| Learning outcomes |
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Basic metods of molecular biology, DNA and proteins izolation and their analysis, bacterial transformation.
Student is able to (after attending the course): - Apply the laboratory skills in molecular biology research. - Perform the basic molecular techniques. - Write properly research reports aimed at solving the given problems and discuss the results |
| Prerequisites |
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unspecified
KBB/BZP ----- or ----- KFC/BZP1 and KBB/MBIO1 |
| Assessment methods and criteria |
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Student performance
100% attendance, protocols´ elaboration, 70% test |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology (2021) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Summer |