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Lecturer(s)
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Poulíčková Aloisie, prof. RNDr. CSc.
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Hašler Petr, doc. RNDr. Ph.D.
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Dvořák Petr, doc. Mgr. Ph.D.
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Course content
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Course for hydrobiologists and phycologists workin on phycological diplomat theses. Detail studies of individual taxonomical groups and their life cycles. Complex approach to study of diversity. Aseptic work, facilities in phycological lab, algal culturing, culture media, cultures, isolation, calibration of the microscope, SEM, TEM, fluorescence, confocal microscopy, kvantitative methods ? abundance, biovolume, optical density, chlorophyl a measurements, fixation, permanent slides, staining, drawing and microphotos, mounting of plates, work with sequence data.
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Learning activities and teaching methods
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Lecture, Observation, Demonstration, Projection (static, dynamic), Training in job and motor Skils, Laboratory Work
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Learning outcomes
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Course focuses on special technics for algal studies, including preparation and culture technics. Students will trein the methods from preparation of media, aseptic work, isolations under the light microscope to drawing and plate mounting.
The aim of this course is to acquire basic skills in field technique - sampling, conservation, preparation. Student is able to obtain unialgal cultures for future use (DNA isolation). Student is able to document finding via indian ink drawing and prepare figure plate for publication.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Didactic Test, Seminar Work
80% of presence in practicals,comparison of kvantitative methods - protocol, mounting of joing figure plate, test + identification of technichs
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Recommended literature
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Andersen, R. A. (2005). Algal culturing techniques. Elsevier Academic press.
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Flügel E (ed.). (2011). Fossil Algae: Recent Results and Developments. Springer, Berlin.
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Hindák, F. (ed.). (1978). Sladkovodné riasy. SPN Bratislava.
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Lund, H.C., Lund J.W.G. (1995). Freshwater Algae. Their microscopic world Explorer.. Biopress Ltd.
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Poulíčková, A., Jurčák, J. (2001). Malý obrazový atlas našich sinic a řas. Vydavatelství UP Olomouc.
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Poulíčková,A., Dvořák P., Hašler. P. (2015). Průvodce mikrosvětem sinic a řas. VUP Olomouc.
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Poulíčková,A. (2011). Základy ekologie sinic a řas. Olomouc.
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Richmond A, Hu Q. (2013). Handbook of Microalgal Culture: Applied Phycology and Biotechnology.. Wiley-Blackwell, Chichester.
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Tebbani S, Filali R, Lopes F, Dumur D, Pareau D. (2014). CO2 Biofixation by Microalgae:Automation Process. Wiley-ISTE, Chichester.
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Yang Z. (2014). Molecular Evolution: A Statistical Approach. Oxford University Press.
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