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Lecturer(s)
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Petr Jan, doc. RNDr. Ph.D.
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Course content
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1. Microscopy, fluorescence microscopy (chemical reactions, immunochemical staining, reconstruction), histochemistry 2. Flow cytometry, laser microdissection 3. Atomic force microscopy, scanning probe microscopy, Raman spectrometry, mechanical nanoscale operations 4. Sensors, microsensors, nanopores, stochastic sensing 5. Micrototal analytical systems (microTAS), lab-on-a-chip, microfluidics, physics of microsystems 6. Microfluidic devices - materials and technologies 7. Microfluidic devices - hydrodynamic and electrokinetic flow, injection, separation 8. Microfluidic devices - detection, mass spectrometry, regulation 9. Applications of microfludic devices - droplet chemistry, PCR, sequenators, proteomics 10. Applications of microfludic devices - diagnostics, immunochemistry 11. Low cost chips (paper technology), electrowetting on dielectrics, digital microfluidics 12. Nanoparticles and microparticles in analytical chemistry
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
- Attendace
- 26 hours per semester
- Preparation for the Exam
- 34 hours per semester
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Learning outcomes
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The aim of this course is to introduce to students modern microscale analytical techniques and miniaturized analytical techniques.
to describe basic principles of miniaturized techniques in analytical chemistry and their application possibilities
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Prerequisites
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The knowledge of basics of analytical chemistry and instrumental methods of analytical chemistry is recommended.
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Assessment methods and criteria
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Written exam
The examination is done by a test (minimally 60 % points should be correct) and by an oral colloquium on two optional themes.
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Recommended literature
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A. Folch. How the World Flows: Microfluidics from Raindrops to Covid Tests. Oxford University Press 2025.
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A. Folch. Introduction to BioMEMS. CRC Press 2012..
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A. Manz, P. Neužil, J.S. O?Connor, S. Simone. Microfluidics and Lab-on-a-chip. RSC 2020..
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C. H. Li. (2005). Microfluidic Lab-on-a-Chip for Chemical and Biological Analysis and Discovery.
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J. P. Landers. (2007). Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques.
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P. Tabeling. (2006). Introduction to Microfluidics.
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Z. El Rassi. Advances in Microscale Electro- and Liquid Phase-Based Separation Techniques. Elsevier 2026.
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