Course: Clinical Analytical Chemistry

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Course title Clinical Analytical Chemistry
Course code ACH/KLACH
Organizational form of instruction Lecture + Seminar
Level of course Master
Year of study not specified
Semester Winter
Number of ECTS credits 4
Language of instruction Czech
Status of course Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Petr Jan, doc. RNDr. Ph.D.
  • Vítková Kateřina, Mgr. Ph.D.
Course content
1. Introduction to clinical analytical chemistry, pathological states of organisms, analytical chemistry in clinical applications, types of examinations 2. Automation, laboratory certifications, quality management, point-of-care testing 3. Biological variability of samples, preanalytical phase, sampling, and sample preparation for different types of examinations 4. Clinical studies, legislation, the role of analytical chemistry 5. Immunological tests, molecular genetics diagnostics, microbiology, hematological tests, blood types, basic principles of histology 6. Introduction to clinical biochemistry - analysis of amino acids, proteins in diagnostics, electrophoresis of proteins, analysis of clinically important proteins 7. Enzymes, enzyme activity, clinically important enzymes, and their determination 8. Carbohydrates, determination of clinically important carbohydrates, function of lipids, analysis of lipids 9. Liver function, markers of metabolism action in liver, renal function, clearance, determination of non-protein nitrogen compounds, clinical applications 10. Electrolytes, determination of ions, regulation of pH, disorders of acid-base balance 11. Hormones, analytics of hormones, regulation of function of muscles, cardiac markers, cancer, tumor markers 12. Analytical chemistry in clinical pharmacology, pharmacokinetics, pharmacodynamics, therapeutic drug monitoring, analytical methods in the European Pharmacopoeia Seminars: Statistical evaluation in clinical chemistry, references, certification of clinical laboratories, information systems, screening tests, interpretation - markers, and their monitoring during disease and during therapy, interpretation of clinical data, case studies, practical examples of problems in the field of clinical analytical chemistry

Learning activities and teaching methods
Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming)
  • Attendace - 52 hours per semester
  • Preparation for the Exam - 35 hours per semester
  • Preparation for the Course Credit - 20 hours per semester
  • Homework for Teaching - 13 hours per semester
Learning outcomes
Modern analytical techniques used in clinical laboratories (e.g., laboratories of clinical chemistry or clinical biochemistry), specifics of the work in clinical laboratories, and the data interpretation are introduced to students.
Students will be able to describe basic principles of analytical techniques used in clinical analytical chemistry and clinical biochemistry, describe, and propose methods for analysis of markers of selected disorders, and do the interpretation of obtained results.
Prerequisites
The knowledge of bioanalytical methods and instrumental techniques of analytical chemistry is highly recommended.

Assessment methods and criteria
Written exam

The credit is given based on a written essay where students will propose a solution of a given problem from the field of clinical analytical chemistry. The examination is done by a written test; minimally 60 % of points should be obtained for passing.
Recommended literature
  • Brodská H. L., Kohout P. a kol. (2022). Laboratorní vyšetření v klinické praxi. Grada, Praha.
  • Chromý V. a kol. (2011). Bioanalytika: Analytické metody v klinické chemii a laboratorní medicíně. Masarykova univerzita, Brno.
  • Marshall W.J., Lapsley M., Day A.P., Ayling R.M. (2014). Clinical Biochemistry: Metabolic and Clinical Aspects. Churchill Livingstone.
  • Nessar A. (2017). Clinical Biochemistry. Oxford University Press.
  • Racek J., Rajdl D. a kol. (2021). Klinická biochemie. Galén, Praha.
  • Šebek J. (2022). Hematologie a laboratorní technika. Galén, Praha.
  • Zima T. a kol. (2013). Laboratorní diagnostika. Galén, Praha.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Analytical Chemistry (2021) Category: Chemistry courses - Recommended year of study:-, Recommended semester: Winter