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Lecturer(s)
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Mrázek František, prof. MUDr. Ph.D.
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Kriegová Eva, doc. Ing. Dr.
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Heřmanová Zuzana, MUDr. Ph.D.
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Weigl Evžen, prof. MUDr. CSc.
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Braunová Jaroslava, MUDr. Ph.D.
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Křupka Michal, Mgr. Ph.D.
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Raška Milan, prof. Mgr. MUDr. Ph.D.
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Hutyrová Beáta, MUDr. Ph.D.
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Course content
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Structure of the primary and secondary lymphatic organs. Cell elements involved in non-specific and antigen specific immune response. Antigen presenting cells dendritic cells, macrophages. Relationship between cell of the innate and antigen specific immunity. Humoral factors of innate and antigen specific defense in systemic, mucosal and skin immunity. Complement. Antibodies - concentration, function, subclasses, B cells activation, primary and secondary response, genetic base of antibody diversity. MHC function, hereditability, biological sense of MHC restriction. Cytokines, chemokines, adhesion molecules. T-cells, CD4 (Th, Treg), CD8 -biological sense, functions. NK cells, B1 cells, gama delta T cells. Investigation of the patient with suspected allergic/immunological disorder - sampling of the patients. Antibodies in laboratory methods, laboratory tests for evaluation of humoral and cell-mediated immunity - methods, reference values, indication, interpretation. HLA-typing - methods, indication, interpretation. Genotyping tests for clinical immunology - PCR, Sanger sequencing, NGS-based tests - principles, differences. Principles laboratory test for allergic patient.
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Learning activities and teaching methods
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Monologic Lecture(Interpretation, Training), Dialogic Lecture (Discussion, Dialog, Brainstorming), Observation, Demonstration, Training in job and motor Skils, Laboratory Work
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Learning outcomes
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Introduction of principles of functioning of the immune system, inherited and antigen specific humoral and cell-mediated immunity, structure of lymphatic system, mechanisms of immune response induction including participating cell populations and signaling, genetic basis of antigen specific immune response diversity, HLA molecules, central and peripheral tolerance, mechanisms of induction and regulation of inflammatory response. Explanation of the differences in functioning of systemic and mucosal immune responses, mechanisms of immune defense to infection and surveillance of tumor development. Principles of hypersensitivity reactions and clinical examples. Introduction to laboratory immunological investigation, including allergen hypersensitivity investigation, principles of specimens collection from patients, principles of clinical immunology laboratory methods, overview about immunology parameters routinely investigated, indications and interpretation. Further, it will be introduced HLA polymorphism typing and selected molecular biology methods for investigation in patients with autoimmunity disorders, immune efficiencies or malignancies.
Students will get an overview about principles of immune system, immune surveillance of infection and tumor, basic principles of immune hypersensitivity. Will understand to the uniqueness and collaboration of systemic and mucosal immune systems. Further, student will get an overview about clinical immunology laboratory methods, immunology parameters routinely investigated, indications and interpretation.
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Prerequisites
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Completion all of prescribed exams.
BIO/VCB11
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Assessment methods and criteria
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Didactic Test
Attendance on seminars and practical training.
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Recommended literature
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Hořejší, V., Bartůňková, J. (2017). Základy imunologie. Triton Praha.
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Raška, M. a kol. (2016). Imunologické vyšetřovací metody pro studenty LF UP v Olomouci. Olomouc: Univerzita Palackého v Olomouci.
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Raška, M. a kol. (2017). Vyšetřovací metody v laboratorní imunologii pro studenty LF UP v Olomouci. Olomouc: VUP.
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