Course: Structural Protein Bioinformatics

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Course title Structural Protein Bioinformatics
Course code KFC/PGSS
Organizational form of instruction Lecture + Seminar
Level of course Doctoral
Year of study not specified
Semester Winter and summer
Number of ECTS credits 20
Language of instruction Czech, English
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Berka Karel, prof. RNDr. Ph.D.
Course content
In the past 10 years the amount of data, stored in the greatest structural database, has risen in one order and the amount of sequential data has risen even in two orders. Structural bioinformatics is a field of study of these structural data towards biological scope. 1. Indroduction, structure to function relationships 2. Protein structure basics - levels of structure, physico-chemical properties of aminoacids and nucleic acids, rotameric libraries, Molecular interactions 3. Terciární struktura proteinů, proteinová architektura a topologie, protein evolution 4. Nucleic acids structure basics - levels of structure 5. Experimental structure elucidation, NMR vs X-ray vz Cryo-EM, resolution and structure validation 6. Structural bioinformatics databases 7. Structure alignment, RMSD, superposition algorithms, DALI, MAMMOTH 8. Structure prediction - homology modelling, threading, ab initio, covariance 9. Function prediction - surface, active site, channels, disorder, membrane, ligand positions 10. Protein-protein and protein-nucleic acid interactions

Learning activities and teaching methods
Monologic Lecture(Interpretation, Training)
  • Preparation for the Exam - 0 hours per semester
Learning outcomes
This course of structural bioinformatics focuses on the current results and procedures.
ability to evaluate particular methods and procedures, explain results related to the structural bioinformatics of proteins
Prerequisites
unspecified

Assessment methods and criteria
Oral exam

Recommended literature
  • Bourne P. & Helge W. (Eds.). (2003). Structural Bioinformatics. Wiley.
  • Eidhammer I., Jonassen I., & Taylor WR. (2004). Protein Bioinformatics - An Algorithmic Approach to Sequence and Structure Analysis. Wiley.
  • Hinchliffe A. (2003). Molecular Modelling for Beginners. Wiley.
  • Leach AR. (2001). Molecular Modelling - Principles and Applications (2 edition). Pearson Education.
  • Orengo CA., Jones DT., Thornton JM. (Eds.). (2003). Bioinformatics - Genes, Proteins and Computers. Bios Scientific Publishers Ltd.
  • Voet D. & Voet JG. (2004). Biochemistry (3rd edition). Wiley.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester