|
Lecturer(s)
|
-
Rulík Martin, doc. RNDr. Ph.D.
-
Buriánková Iva, Mgr. Ph.D.
|
|
Course content
|
Introduction and basic terminology, ecological interactions between microorganisms and their environment, methods of DNA/RNA extraction from environmental samples, PCR and RT-PCR, sequencing analysis, phylogenetic analysis, DNA fingerprinting of microbial community - DGGE, TGGE, SSCP, T-RFLP, AFLP, nucleic acids hybridization (whole-cell FISH, microarrays). Gene cloning, microbial ecology and genomics, metagenomic libraries of non-cultivated organisms, bioinformatics and web databases in microbial ecology.
|
|
Learning activities and teaching methods
|
|
Lecture, Monologic Lecture(Interpretation, Training), Demonstration
|
|
Learning outcomes
|
Lecture provides basic overview of biology and ecology of microorganisms in environmental habitats and possible using of molecular methods for their study. While focusing on description of microbial diversity and construction of phylogenetic trees based on the 16S rRNA similarity. Main attempt is put on understanding of advantages of various molecular methods and approaches used in the microbiological research.
At the end of a semester it is expected that student will: - have deepened knowledge on occurrence, diversity and role of microorganisms in the nature - know about possible using of molecular methods in microbial ecology - be able to intepret results of varous studies aimed with detection of microorganisms by using molecular methods
|
|
Prerequisites
|
Elementary knowledhe in microbiology, ecology, molecular and cell biology.
|
|
Assessment methods and criteria
|
Mark, Oral exam
Oral exam.
|
|
Recommended literature
|
-
Brown TA (2007). Klonování a analýza DNA. Univerzita Palackého v Olomouci, 389 pp.,.
-
De Bruijn FJ (ed). (2011). Handbook of molecular microbial ecology I: Metogenomics and complementary approaches.. New Jersey.
-
De Bruijn FJ (ed). (2011). Handbook of molecular microbial ecology II: Metogenomics in different habitats..
-
Madigan MT, Martinko JM (2006). Brock biology of microorganisms, 11th ed. (nebo pozdější vydání). Pearson Education, Inc., Upper Saddle River, NJ,.
-
Oren A, Papke RT (2010). Molecular phylogeny of microorganisms. Caister Academic Press, Norfolk, UK,.
-
Osborn M, Smith C (2005)(eds). Molecular microbial ecology. Taylor & Francis, New York, NY.
-
Rulík M, Buriánková I, Brablcová L. (2015). Vybrané kapitoly z molekulární ekologie mikroorganizmů. Olomouc.
|